Re-analysis of the Human Transcription Factor Atlas Recovers TF-Specific Signatures from Pooled Single-Cell Screens with Missing Controls
Public pooled single-cell perturbation atlases are valuable resources for studying transcription factor (TF) function, but downstream re-analysis can be limited by incomplete deposited metadata and missing internal controls. Here we re-analyze the human TF Atlas dataset (GSE216481), a MORF-based pooled overexpression screen spanning 3,550 TF open reading frames and 254,519 cells, with a reproducible pipeline for quality control, MORF barcode demultiplexing, per-TF differential expression, and functional enrichment. From 77,018 cells in the pooled screen, we assign 60,997 (79.2\%) to 87 TF identities. Because the deposited barcode mapping lacks the GFP and mCherry negative controls present...
| Authors | Arka Jain, Umesh Sharma |
|---|---|
| Primary category | cs.LG |
| Published | 2 April 2026 |
| Last updated at source | 16 May 2026 |
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